We used Ca(2+) imaging to monitor areas located within the ventral spinal horn at 1 and 2 weeks of in vitro growth. Primitive patterns of spontaneous neuronal Ca(2+) transients (detected at 1 week) were typically synchronous. Remarkably, such transients originated from widespread propagating waves that became organized into large-scale rhythmic bursts. These activities were associated with the generation of synaptically mediated inward currents under whole-cell patch-clamp. Such patterns disappeared during longer culture of spinal segments: at 2 weeks in culture, only a subset of ventral neurons displayed spontaneous, asynchronous and repetitive Ca(2+) oscillations dissociated from background synaptic activity. We observed that the emergence of oscillations was a restricted phenomenon arising together with the transformation of ventral network electrophysiological bursting into asynchronous synaptic discharges.

The patterns of spontaneous Ca2+ signals generated by ventral spinal neurons in vitro show time-dependent refinement / Sibilla, S.; Fabbro, A.; Grandolfo, M.; D'Andrea, P.; Nistri, A.; Ballerini, L. - In: EUROPEAN JOURNAL OF NEUROSCIENCE. - ISSN 0953-816X. - 29:8(2009), pp. 1543-1559. [10.1111/j.1460-9568.2009.06708.x]

The patterns of spontaneous Ca2+ signals generated by ventral spinal neurons in vitro show time-dependent refinement

Fabbro, A.;Grandolfo, M.;Nistri, A.;Ballerini, L
2009-01-01

Abstract

We used Ca(2+) imaging to monitor areas located within the ventral spinal horn at 1 and 2 weeks of in vitro growth. Primitive patterns of spontaneous neuronal Ca(2+) transients (detected at 1 week) were typically synchronous. Remarkably, such transients originated from widespread propagating waves that became organized into large-scale rhythmic bursts. These activities were associated with the generation of synaptically mediated inward currents under whole-cell patch-clamp. Such patterns disappeared during longer culture of spinal segments: at 2 weeks in culture, only a subset of ventral neurons displayed spontaneous, asynchronous and repetitive Ca(2+) oscillations dissociated from background synaptic activity. We observed that the emergence of oscillations was a restricted phenomenon arising together with the transformation of ventral network electrophysiological bursting into asynchronous synaptic discharges.
2009
29
8
1543
1559
https://doi.org/10.1111/j.1460-9568.2009.06708.x
Sibilla, S.; Fabbro, A.; Grandolfo, M.; D'Andrea, P.; Nistri, A.; Ballerini, L
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.11767/12575
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