We fabricated a nanocomposite substrate made of nanofibers on micropillar arrays by photolithography, soft lithography and electrospinning for cell culture studies. This nanocomposite substrate combines the advantage of the extra-cellular matrix (ECM)-like surface morphology and high porosity and low stiffness of underneath supporting material. For neuronal culture studies, we used nanofibers of poly (lactic-co-glycolic acid) (PLGA) on high aspect ratio micropillars of polydimethylsiloxane (PDMS). Our results showed that primary hippocampal neurons on such a nanocomposite substrate have different cell morphology than on flat surfaces and they showed more electric activities. Thus, the nanofiber-micropillar composite substrates were shown to be useful for neuron culture studies. © 2017

Fabrication of PLGA nanofibers on PDMS micropillars for neuron culture studies / Wei, Jin; Pozzi, Diletta; Ulloa Severino, Francesco Paolo; Torre, Vincent; Chen, Yong. - In: MICROELECTRONIC ENGINEERING. - ISSN 0167-9317. - 175:May(2017), pp. 67-72. [10.1016/j.mee.2017.01.015]

Fabrication of PLGA nanofibers on PDMS micropillars for neuron culture studies

Pozzi, Diletta;Ulloa Severino, Francesco Paolo;Torre, Vincent;
2017-01-01

Abstract

We fabricated a nanocomposite substrate made of nanofibers on micropillar arrays by photolithography, soft lithography and electrospinning for cell culture studies. This nanocomposite substrate combines the advantage of the extra-cellular matrix (ECM)-like surface morphology and high porosity and low stiffness of underneath supporting material. For neuronal culture studies, we used nanofibers of poly (lactic-co-glycolic acid) (PLGA) on high aspect ratio micropillars of polydimethylsiloxane (PDMS). Our results showed that primary hippocampal neurons on such a nanocomposite substrate have different cell morphology than on flat surfaces and they showed more electric activities. Thus, the nanofiber-micropillar composite substrates were shown to be useful for neuron culture studies. © 2017
2017
175
May
67
72
https://doi.org/10.1016/j.mee.2017.01.015
Wei, Jin; Pozzi, Diletta; Ulloa Severino, Francesco Paolo; Torre, Vincent; Chen, Yong
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.11767/50050
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