Optical monitoring of neuronal voltage using fluorescent indicators is a powerful approach for the interrogation of the cellular and molecular logic of the nervous system. Herein, a semisynthetic tethered voltage indicator (STeVI1) based upon nile red is described that displays voltage sensitivity when genetically targeted to neuronal membranes. This environmentally sensitive probe allows for wash-free imaging and faithfully detects supra- and sub-threshold activity in neurons.
A Chemogenetic Approach for the Optical Monitoring of Voltage in Neurons / Sundukova, Mayya; Prifti, Efthymia; Bucci, Annalisa; Kirillova, Kseniia; Serrao, Joana; Reymond, Luc; Umebayashi, Miwa; Hovius, Ruud; Riezman, Howard; Johnsson, Kai; Heppenstall, Paul A.. - In: ANGEWANDTE CHEMIE. INTERNATIONAL EDITION. - ISSN 1433-7851. - 58:8(2019), pp. 2341-2344. [10.1002/anie.201812967]
A Chemogenetic Approach for the Optical Monitoring of Voltage in Neurons
Sundukova, Mayya;Heppenstall, Paul A.
2019-01-01
Abstract
Optical monitoring of neuronal voltage using fluorescent indicators is a powerful approach for the interrogation of the cellular and molecular logic of the nervous system. Herein, a semisynthetic tethered voltage indicator (STeVI1) based upon nile red is described that displays voltage sensitivity when genetically targeted to neuronal membranes. This environmentally sensitive probe allows for wash-free imaging and faithfully detects supra- and sub-threshold activity in neurons.| File | Dimensione | Formato | |
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