A method for determining the ability of antibody to bind to antigen in an intracellular environment comprises (a) providing first and second molecules which interact to generate a signal; (b) providing an intracellular antibody associated with the first molecule and an intracellular antigen associated with the second molecule; (c) assessing the intracellular Ab/Ag interaction by monitoring the signal generated; and, optionally, (d) isolating those antibodies which give rise to a signal. The antibody may be an intact immunoglobulin or a Fv, scFv, Fab or F(ab')<SB>2</SB> fragment. The first molecule may particularly be the activation domain of VP16 and the second molecule may be the binding domain of LexA.

Selection of Intracellular Immunoglobulins / Rabbits, T. H.; Tse, E.; Cattaneo, A.; Visintin, M.. - (1999 Mar 10).

Selection of Intracellular Immunoglobulins

Cattaneo, A.;Visintin, M.
1999-03-10

Abstract

A method for determining the ability of antibody to bind to antigen in an intracellular environment comprises (a) providing first and second molecules which interact to generate a signal; (b) providing an intracellular antibody associated with the first molecule and an intracellular antigen associated with the second molecule; (c) assessing the intracellular Ab/Ag interaction by monitoring the signal generated; and, optionally, (d) isolating those antibodies which give rise to a signal. The antibody may be an intact immunoglobulin or a Fv, scFv, Fab or F(ab')2 fragment. The first molecule may particularly be the activation domain of VP16 and the second molecule may be the binding domain of LexA.
10-mar-1999
GB19990005510 19990310
Rabbits, T. H.; Tse, E.; Cattaneo, A.; Visintin, M.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.11767/88878
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