A method for determining the ability of antibody to bind to antigen in an intracellular environment comprises (a) providing first and second molecules which interact to generate a signal; (b) providing an intracellular antibody associated with the first molecule and an intracellular antigen associated with the second molecule; (c) assessing the intracellular Ab/Ag interaction by monitoring the signal generated; and, optionally, (d) isolating those antibodies which give rise to a signal. The antibody may be an intact immunoglobulin or a Fv, scFv, Fab or F(ab')<SB>2</SB> fragment. The first molecule may particularly be the activation domain of VP16 and the second molecule may be the binding domain of LexA.

Selection of Intracellular Immunoglobulins / Rabbits, T. H.; Tse, E.; Cattaneo, A.; Visintin, M.. - (1999 Mar 10).

Selection of Intracellular Immunoglobulins

Cattaneo, A.;Visintin, M.
1999-03-10

Abstract

A method for determining the ability of antibody to bind to antigen in an intracellular environment comprises (a) providing first and second molecules which interact to generate a signal; (b) providing an intracellular antibody associated with the first molecule and an intracellular antigen associated with the second molecule; (c) assessing the intracellular Ab/Ag interaction by monitoring the signal generated; and, optionally, (d) isolating those antibodies which give rise to a signal. The antibody may be an intact immunoglobulin or a Fv, scFv, Fab or F(ab')2 fragment. The first molecule may particularly be the activation domain of VP16 and the second molecule may be the binding domain of LexA.
GB19990005510 19990310
Rabbits, T. H.; Tse, E.; Cattaneo, A.; Visintin, M.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/20.500.11767/88878
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