Whether new neurons are added in the postnatal cerebral cortex is still debated. Here, we report that the meninges of perinatal mice contain a population of neurogenic progenitors formed during embryonic development that migrate to the caudal cortex and differentiate into Satb2+ neurons in cortical layers II–IV. The resulting neurons are electrically functional and integrated into local microcircuits. Single-cell RNA sequencing identified meningeal cells with distinct transcriptome signatures characteristic of (1) neurogenic radial glia-like cells (resembling neural stem cells in the SVZ), (2) neuronal cells, and (3) a cell type with an intermediate phenotype, possibly representing radial glia-like meningeal cells differentiating to neuronal cells. Thus, we have identified a pool of embryonically derived radial glia-like cells present in the meninges that migrate and differentiate into functional neurons in the neonatal cerebral cortex.

Neurogenic Radial Glia-like Cells in Meninges Migrate and Differentiate into Functionally Integrated Neurons in the Neonatal Cortex / Bifari, F.; Decimo, I.; Pino, A.; Llorens-Bobadilla, E.; Zhao, S.; Lange, C.; Panuccio, G.; Boeckx, B.; Thienpont, B.; Vinckier, S.; Wyns, S.; Bouche, A.; Lambrechts, D.; Giugliano, M.; Dewerchin, M.; Martin-Villalba, A.; Carmeliet, P.. - In: CELL STEM CELL. - ISSN 1934-5909. - 20:3(2017), pp. 360-373.e7. [10.1016/j.stem.2016.10.020]

Neurogenic Radial Glia-like Cells in Meninges Migrate and Differentiate into Functionally Integrated Neurons in the Neonatal Cortex

Giugliano, M.;
2017

Abstract

Whether new neurons are added in the postnatal cerebral cortex is still debated. Here, we report that the meninges of perinatal mice contain a population of neurogenic progenitors formed during embryonic development that migrate to the caudal cortex and differentiate into Satb2+ neurons in cortical layers II–IV. The resulting neurons are electrically functional and integrated into local microcircuits. Single-cell RNA sequencing identified meningeal cells with distinct transcriptome signatures characteristic of (1) neurogenic radial glia-like cells (resembling neural stem cells in the SVZ), (2) neuronal cells, and (3) a cell type with an intermediate phenotype, possibly representing radial glia-like meningeal cells differentiating to neuronal cells. Thus, we have identified a pool of embryonically derived radial glia-like cells present in the meninges that migrate and differentiate into functional neurons in the neonatal cerebral cortex.
20
3
360
373.e7
https://doi.org/10.1016/j.stem.2016.10.020
Bifari, F.; Decimo, I.; Pino, A.; Llorens-Bobadilla, E.; Zhao, S.; Lange, C.; Panuccio, G.; Boeckx, B.; Thienpont, B.; Vinckier, S.; Wyns, S.; Bouche, A.; Lambrechts, D.; Giugliano, M.; Dewerchin, M.; Martin-Villalba, A.; Carmeliet, P.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/20.500.11767/102940
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