The spliceosome, a protein-directed metallo-ribozyme, catalyzes premature mRNA splicing via two transesterification reactions. The atomic-level details of the splicing mechanism and the role of the entwined protein-RNA environment during catalysis remain unresolved. Here, quantum-classical molecular dynamics simulations along with thermodynamic integration unveil that the second catalytic (exon-ligation) step occurs via an associative two-Mg2+-ion mechanism, exclusively catalyzed by RNA, with the scissile phosphate mediating a proton transfer from the nucleophile to the leaving group. Our outcomes provide fundamental advances in understanding the splicing mechanism in eukaryotes disclosing how the catalytic core of spliceosome's ancestors self-splicing ribozymes remained conserved during evolution.

All-Atom Simulations Decrypt the Molecular Terms of RNA Catalysis in the Exon-Ligation Step of the Spliceosome / Borisek, J.; Magistrato, A.. - In: ACS CATALYSIS. - ISSN 2155-5435. - 10:9(2020), pp. 5328-5334. [10.1021/acscatal.0c00390]

All-Atom Simulations Decrypt the Molecular Terms of RNA Catalysis in the Exon-Ligation Step of the Spliceosome

Magistrato A.
2020

Abstract

The spliceosome, a protein-directed metallo-ribozyme, catalyzes premature mRNA splicing via two transesterification reactions. The atomic-level details of the splicing mechanism and the role of the entwined protein-RNA environment during catalysis remain unresolved. Here, quantum-classical molecular dynamics simulations along with thermodynamic integration unveil that the second catalytic (exon-ligation) step occurs via an associative two-Mg2+-ion mechanism, exclusively catalyzed by RNA, with the scissile phosphate mediating a proton transfer from the nucleophile to the leaving group. Our outcomes provide fundamental advances in understanding the splicing mechanism in eukaryotes disclosing how the catalytic core of spliceosome's ancestors self-splicing ribozymes remained conserved during evolution.
10
9
5328
5334
Borisek, J.; Magistrato, A.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.11767/116659
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