Parallel single-cell sequencing protocols represent powerful methods for investigating regulatory relationships, including epigenome-transcriptome interactions. Here, we report a single-cell method for parallel chromatin accessibility, DNA methylation and transcriptome profiling. scNMT-seq (single-cell nucleosome, methylation and transcription sequencing) uses a GpC methyltransferase to label open chromatin followed by bisulfite and RNA sequencing. We validate scNMT-seq by applying it to differentiating mouse embryonic stem cells, finding links between all three molecular layers and revealing dynamic coupling between epigenomic layers during differentiation.

scNMT-seq enables joint profiling of chromatin accessibility DNA methylation and transcription in single cells / Clark, Sj; Argelaguet, R; Kapourani, Ca; Stubbs, Tm; Lee, Hj; Alda-Catalinas, C; Krueger, F; Sanguinetti, G; Kelsey, G; Marioni, Jc; Stegle, O; Reik, W. - In: NATURE COMMUNICATIONS. - ISSN 2041-1723. - 9:(2018), pp. 1-9. [10.1038/s41467-018-03149-4]

scNMT-seq enables joint profiling of chromatin accessibility DNA methylation and transcription in single cells

Sanguinetti G;
2018-01-01

Abstract

Parallel single-cell sequencing protocols represent powerful methods for investigating regulatory relationships, including epigenome-transcriptome interactions. Here, we report a single-cell method for parallel chromatin accessibility, DNA methylation and transcriptome profiling. scNMT-seq (single-cell nucleosome, methylation and transcription sequencing) uses a GpC methyltransferase to label open chromatin followed by bisulfite and RNA sequencing. We validate scNMT-seq by applying it to differentiating mouse embryonic stem cells, finding links between all three molecular layers and revealing dynamic coupling between epigenomic layers during differentiation.
2018
9
1
9
781
Clark, Sj; Argelaguet, R; Kapourani, Ca; Stubbs, Tm; Lee, Hj; Alda-Catalinas, C; Krueger, F; Sanguinetti, G; Kelsey, G; Marioni, Jc; Stegle, O; Reik, ...espandi
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.11767/117082
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