A series of prion transmission experiments was performed in transgenic (Tg) mice expressing either wild-type, chimeric, or truncated prion protein (PrP) molecules. Following inoculation with Rocky Mountain Laboratory (RML) murine prions, scrapie incubation times for Tg(MoPrP)4053, Tg(MHM2)294/Prnp(0/0), and Tg(MoPrP, Delta23-88)9949/Prnp(0/0) mice were approximately 50, 120, and 160 days, respectively. Similar scrapie incubation times were obtained after inoculation of these lines of Tg mice with either MHM2(MHM2(RML)) or MoPrP(Delta23-88)(RML) prions, excluding the possibility that sequence-dependent transmission barriers could account for the observed differences. Tg(MHM2)294/Prnp(0/0) mice displayed prolonged scrapie incubation times with four different strains of murine prions. These data provide evidence that the N terminus of MoPrP and the chimeric region of MHM2 PrP (residues 108 through 111) both influence the inherent efficiency of prion propagation.
|Titolo:||Identification of two prion protein regions that modify scrapie incubation time|
|Autori:||Supattapone, S.; Muramoto, T.; Legname, G.; Mehlhorn, I.; Cohen, F. E.; Dearmond, S. J.; Prusiner, S. B.; Scott, M. R.|
|Data di pubblicazione:||2001|
|Digital Object Identifier (DOI):||10.1128/JVI.75.3.1408-1413.2001|
|Appare nelle tipologie:||1.1 Journal article|