The T lymphocyte-specific protein tyrosine kinase p56lck (Lck) is an essential component of the TCR-mediated signal transduction complex. Lck knockout mice have reduced numbers of double-positive thymocytes and very few mature single-positive cells, particularly of the CD4 lineage. Here we demonstrate the ability of a tetracycline-based tissue-specific inducible Lck transgene to restore expansion of early thymocytes and maturation of single-positive cells in Lckneg mice upon induction with doxycycline. Restoration of Lck expression is particularly important for positive selection to the CD4+ lineage but has a lesser impact on selection to the CD8+ lineage, suggesting activation of Lck is an important component of the signals involved in lineage choice during thymic differentiation.
|Titolo:||Inducible expression of a p56Lck transgene reveals a central role for Lck in the differentiation of CD4 SP thymocytes|
|Autori:||Legname, Giuseppe; Seddon, B; Lovatt, M; Tomlinson, P; Sarner, N; Tolaini, M; Williams, K; Norton, T; Kioussis, D; Zamoyska, R.|
|Data di pubblicazione:||2000|
|Digital Object Identifier (DOI):||10.1016/S1074-7613(00)80205-8|
|Fulltext via DOI:||https://doi.org/10.1016/S1074-7613(00)80205-8|
|Appare nelle tipologie:||1.1 Journal article|