We used Ca(2+) imaging to monitor areas located within the ventral spinal horn at 1 and 2 weeks of in vitro growth. Primitive patterns of spontaneous neuronal Ca(2+) transients (detected at 1 week) were typically synchronous. Remarkably, such transients originated from widespread propagating waves that became organized into large-scale rhythmic bursts. These activities were associated with the generation of synaptically mediated inward currents under whole-cell patch-clamp. Such patterns disappeared during longer culture of spinal segments: at 2 weeks in culture, only a subset of ventral neurons displayed spontaneous, asynchronous and repetitive Ca(2+) oscillations dissociated from background synaptic activity. We observed that the emergence of oscillations was a restricted phenomenon arising together with the transformation of ventral network electrophysiological bursting into asynchronous synaptic discharges.
|Titolo:||The patterns of spontaneous Ca2+ signals generated by ventral spinal neurons in vitro show time-dependent refinement|
|Autori:||SIBILLA S; FABBRO A; GRANDOLFO M; D'ANDREA P; NISTRI A; BALLERINI L|
|Data di pubblicazione:||2009|
|Digital Object Identifier (DOI):||10.1111/j.1460-9568.2009.06708.x|
|Appare nelle tipologie:||1.1 Journal article|