We investigated the expression of ERG channels in immature spinal interneurons, using organotypic embryonic cultures of mouse spinal cord after 1 and 2 weeks of development in vitro. We report here that all the genes of the erg family known so far (erg1a,erg1b, erg2, erg3) are expressed in embryonic spinal cultures. We demonstrate for the first time that three ERG proteins (ERG1A, ERG2 and ERG3) are co-expressed in the same neuronal population, and display a spatio-temporal distribution in the spinal slices. ERG immuno-positive cells, representing mainly GABAergic interneurons, were present in large numbers at early stages of development, while declining later, with a ventral to dorsal gradient. Patch clamp recordings confirmed these data, showing that ventral interneurons expressed functional ERG currents only transiently. Similar expression of the erg genes was observed at comparable ages in vivo. The role of ERG currents in regulating neuronal excitability during the earliest phases of spinal circuitry development will be examined in future studies.
|Titolo:||The HERG-current is transiently expressed during development of mouse spinal network in vitro|
|Autori:||Furlan, F.; Guasti, L.; Avossa, D.; Becchetti, A.; Cilia, E.; Ballerini, L.; Arcangeli, A.|
|Data di pubblicazione:||2005|
|Digital Object Identifier (DOI):||10.1016/j.neuroscience.2005.06.040|
|Appare nelle tipologie:||1.1 Journal article|