The rodent vomeronasal organ plays an important role in many social behaviors. Using the calcium imaging technique with the dye fluo-4 we measured intracellular calcium concentration changes induced by the application of sulfated steroids to neurons isolated from the vomeronasal organ of female mice. We found that a mix of 10 sulfated steroids from the androgen, estrogen, pregnanolone, and glucocorticoid families induced a calcium response in 71% of neurons. Moreover, 31% of the neurons responded to a mix composed of 3 glucocorticoid-derived compounds, and 28% responded to a mix composed of 3 pregnanolone-derived compounds. Immunohistochemistry showed that neurons responding to sulfated steroids expressed phosphodiesterase 4A, a marker specific for apical neurons expressing V1R receptors. None of the neuron that responded to 1 mix responded also to the other, indicating a specificity of the responses. Some neurons responded to more than 1 individual component of the glucocorticoid-derived mix tested at high concentration, suggesting that these neurons are broadly tuned, although they still displayed strong specificity, remaining unresponsive to high concentrations of the ineffective compounds. © The Authors 2012. Published by Oxford University Press. All rights reserved.
|Titolo:||Responses to sulfated steroids of female mouse vomeronasal sensory neurons|
|Autori:||Celsi, F.; D'Errico, A.; Menini, A.|
|Data di pubblicazione:||2012|
|Digital Object Identifier (DOI):||10.1093/chemse/bjs068|
|Fulltext via DOI:||10.1093/chemse/bjs068|
|Appare nelle tipologie:||1.1 Journal article|