In neuroinflammation, astrocytes play multi-faceted roles that regulate the neuronal environment. Astrocytes sense and respond to pro-inflammatory cytokines (CKs) and, by a repertoire of intracellular Ca2+ signaling, contribute to disease progression. Therapeutic approaches wish to reduce the overactivation in Ca2+ signaling in inflammatory-reactive astrocytes to restore dysregulated cellular changes. Cell-targeting therapeutics might take advantage by the use of nanomaterial-multifunctional platforms such as graphene oxide(GO). GO biomedical applications in the nervous system involve therapeutic delivery and sensing, and GO flakes were shown to enable interfacing of neuronal and glial membrane dynamics. We exploit organotypic spinal cord cultures and optical imaging to explore Ca2+ changes in astrocytes, and we report, when spinal tissue is exposed to CKs, neuroinflammatory-associated modulation of resident glia. We show the efficacy of GO to revert these dynamic changes in astrocytic reactivity to CKs, and we translate this potential in an animal model of immune-mediated neuroinflammatory disease.

Graphene Oxide Nanosheets Reduce Astrocyte Reactivity to Inflammation and Ameliorate Experimental Autoimmune Encephalomyelitis / Di Mauro, Giuseppe; Amoriello, Roberta; Lozano, Neus; Carnasciali, Alberto; Guasti, Daniele; Becucci, Maurizio; Cellot, Giada; Kostarelos, Kostas; Ballerini, Clara; Ballerini, Laura. - In: ACS NANO. - ISSN 1936-086X. - 17:3(2023), pp. 1965-1978. [10.1021/acsnano.2c06609]

Graphene Oxide Nanosheets Reduce Astrocyte Reactivity to Inflammation and Ameliorate Experimental Autoimmune Encephalomyelitis

Di Mauro, Giuseppe;Amoriello, Roberta;Cellot, Giada
;
Ballerini, Laura
2023-01-01

Abstract

In neuroinflammation, astrocytes play multi-faceted roles that regulate the neuronal environment. Astrocytes sense and respond to pro-inflammatory cytokines (CKs) and, by a repertoire of intracellular Ca2+ signaling, contribute to disease progression. Therapeutic approaches wish to reduce the overactivation in Ca2+ signaling in inflammatory-reactive astrocytes to restore dysregulated cellular changes. Cell-targeting therapeutics might take advantage by the use of nanomaterial-multifunctional platforms such as graphene oxide(GO). GO biomedical applications in the nervous system involve therapeutic delivery and sensing, and GO flakes were shown to enable interfacing of neuronal and glial membrane dynamics. We exploit organotypic spinal cord cultures and optical imaging to explore Ca2+ changes in astrocytes, and we report, when spinal tissue is exposed to CKs, neuroinflammatory-associated modulation of resident glia. We show the efficacy of GO to revert these dynamic changes in astrocytic reactivity to CKs, and we translate this potential in an animal model of immune-mediated neuroinflammatory disease.
2023
17
3
1965
1978
doi.org/10.1021/acsnano.2c06609
Di Mauro, Giuseppe; Amoriello, Roberta; Lozano, Neus; Carnasciali, Alberto; Guasti, Daniele; Becucci, Maurizio; Cellot, Giada; Kostarelos, Kostas; Ballerini, Clara; Ballerini, Laura
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.11767/130890
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