DNA and RNA polymerases active on bacterial and human genomes in the crowded environment of a cell are modeled as beads spaced along a string. Aggregation of the large polymerizing complexes increases the entropy of the system through an increase in entropy of the many small crowding molecules; this occurs despite the entropic costs of looping the intervening DNA. Results of a quantitative cost/benefit analysis are consistent with observations that active polymerases cluster into replication and transcription "factories" in both pro- and eu-karyotes. We conclude the second law of thermodynamics acts through non-specific entropic forces between engaged polymerases to drive the self-organization of genomes into loops containing several thousands (and sometimes millions) of base-pairs.
|Titolo:||Entropy-driven genome organization|
|Autori:||MARENDUZZO D; MICHELETTI C; COOK P.R|
|Data di pubblicazione:||2006|
|Digital Object Identifier (DOI):||10.1529/biophysj.105.077685|
|Appare nelle tipologie:||1.1 Journal article|