Previous immunostaining protocols are highly specific for model organisms and often not suitable for diverse specimens that are non-perfused and over-fixed (i.e., tissues sitting in fixatives for months/year). Here, we present an immunofluorescence protocol for localizing protein targets in brain tissue from 11 model and non-model mammals. We describe preparation of both fresh and fixed tissues including steps for deparaffinization, fixation, and cryoprotection.
Immunofluorescence protocol for localizing protein targets in brain tissue from diverse model and non-model mammals / Ciani, Caterina; Pistorio, Giulio; Mearelli, Marika; Falcone, Carmen. - In: STAR PROTOCOLS. - ISSN 2666-1667. - 4:3(2023), pp. 1-12. [10.1016/j.xpro.2023.102482]
Immunofluorescence protocol for localizing protein targets in brain tissue from diverse model and non-model mammals
Ciani, Caterina;Pistorio, Giulio;Mearelli, Marika;Falcone, Carmen
2023-01-01
Abstract
Previous immunostaining protocols are highly specific for model organisms and often not suitable for diverse specimens that are non-perfused and over-fixed (i.e., tissues sitting in fixatives for months/year). Here, we present an immunofluorescence protocol for localizing protein targets in brain tissue from 11 model and non-model mammals. We describe preparation of both fresh and fixed tissues including steps for deparaffinization, fixation, and cryoprotection.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
