Organotypic cocultures of dorsal root ganglia and spinal cord from embryonic rats provides direct access to spinal interneurons in a culture system in which the cytoarchitectural organization of the spinal cord slice is maintained. This preparation was used to investigate the possible induction of rhythmic behaviour at different times of development in vitro. Spontaneous rhythmic bursts induced by coapplication of strychnine (1 microM) and bicuculline (20 microM) were observed with patch-clamp recordings from ventral interneurons. Ventral horn interneurons consistently developed a very regular pattern of activity which was superimposed on a background of sustained synaptic activity. The pattern of the spontaneous bursting following application of strychnine and bicuculline showed a developmentally regulated difference in frequency between two distinct stages of in vitro development.

Network bursting by organotypic spinal slice cultures in the presence of bicuculline and/or strychnine is developmentally regulated / Ballerini, L.; Galante, M.. - In: EUROPEAN JOURNAL OF NEUROSCIENCE. - ISSN 0953-816X. - 10:9(1998), pp. 2871-2879. [10.1111/j.1460-9568.1998.00296.x]

Network bursting by organotypic spinal slice cultures in the presence of bicuculline and/or strychnine is developmentally regulated.

Ballerini, L.;Galante, M.
1998-01-01

Abstract

Organotypic cocultures of dorsal root ganglia and spinal cord from embryonic rats provides direct access to spinal interneurons in a culture system in which the cytoarchitectural organization of the spinal cord slice is maintained. This preparation was used to investigate the possible induction of rhythmic behaviour at different times of development in vitro. Spontaneous rhythmic bursts induced by coapplication of strychnine (1 microM) and bicuculline (20 microM) were observed with patch-clamp recordings from ventral interneurons. Ventral horn interneurons consistently developed a very regular pattern of activity which was superimposed on a background of sustained synaptic activity. The pattern of the spontaneous bursting following application of strychnine and bicuculline showed a developmentally regulated difference in frequency between two distinct stages of in vitro development.
1998
10
9
2871
2879
https://doi.org/10.1111/j.1460-9568.1998.00296.x
Ballerini, L.; Galante, M.
File in questo prodotto:
File Dimensione Formato  
1998_EJN.pdf

non disponibili

Tipologia: Versione Editoriale (PDF)
Licenza: Non specificato
Dimensione 676.71 kB
Formato Adobe PDF
676.71 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.11767/13925
Citazioni
  • ???jsp.display-item.citation.pmc??? 7
  • Scopus 43
  • ???jsp.display-item.citation.isi??? 41
social impact