3D printing at the micrometer scale enables rapid prototyping of polymeric devices for neuronal cell cultures. As a proof-of-principle, structural connections between neurons were constrained by creating barriers and channels influencing neurite outgrowth, while the functional consequences of such manipulation were observed by extracellular electrophysiology.

Two-Photon Polymerization 3D-Printing of Micro-scale Neuronal Cell Culture Devices / Hosseini, Ali; Noselli, Giovanni; Giugliano, Michele. - In: JOURNAL OF VISUALIZED EXPERIMENTS. - ISSN 1940-087X. - 208:(2024), pp. 1-20. [10.3791/66142]

Two-Photon Polymerization 3D-Printing of Micro-scale Neuronal Cell Culture Devices

Hosseini, Ali;Noselli, Giovanni;Giugliano, Michele
2024-01-01

Abstract

3D printing at the micrometer scale enables rapid prototyping of polymeric devices for neuronal cell cultures. As a proof-of-principle, structural connections between neurons were constrained by creating barriers and channels influencing neurite outgrowth, while the functional consequences of such manipulation were observed by extracellular electrophysiology.
2024
208
1
20
e66142
https://dx.doi.org/10.3791/66142
Hosseini, Ali; Noselli, Giovanni; Giugliano, Michele
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.11767/140090
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