In the last decade, carbon nanotube growth substrates have been used to investigate neurons and neuronal networks formation in vitro when guided by artificial nano-scaled cues. Besides, nanotube-based interfaces are being developed, such as prosthesis for monitoring brain activity. We recently described how carbon nanotube substrates alter the electrophysiological and synaptic responses of hippocampal neurons in culture. This observation highlighted the exceptional ability of this material in interfering with nerve tissue growth. Here we test the hypothesis that carbon nanotube scaffolds promote the development of immature neurons isolated from the neonatal rat spinal cord, and maintained in vitro. To address this issue we performed electrophysiological studies associated to gene expression analysis. Our results indicate that spinal neurons plated on electro-conductive carbon nanotubes show a facilitated development. Spinal neurons anticipate the expression of functional markers of maturation, such as the generation of voltage dependent currents or action potentials. These changes are accompanied by a selective modulation of gene expression, involving neuronal and non-neuronal components. Our microarray experiments suggest that carbon nanotube platforms trigger reparative activities involving microglia, in the absence of reactive gliosis. Hence, future tissue scaffolds blended with conductive nanotubes may be exploited to promote cell differentiation and reparative pathways in neural regeneration strategies.

Adhesion to carbon nanotube conductive scaffolds forces action-potential appearance in immature rat spinal neurons / Fabbro, Alessandra; Sucapane, A.; Toma, Francesca Maria; Calura, E.; Rizzetto, L.; Carrieri, Claudia; Roncaglia, Paola; Martinelli, V.; Scaini, Denis; Masten, L.; Turco, Alessandro; Gustincich, Stefano; Prato, M.; Ballerini, Laura. - In: PLOS ONE. - ISSN 1932-6203. - 8:8(2013), pp. 1-14. [10.1371/journal.pone.0073621]

Adhesion to carbon nanotube conductive scaffolds forces action-potential appearance in immature rat spinal neurons

Fabbro, Alessandra;Toma, Francesca Maria;Carrieri, Claudia;Roncaglia, Paola;Scaini, Denis;Turco, Alessandro;Gustincich, Stefano;Ballerini, Laura
2013

Abstract

In the last decade, carbon nanotube growth substrates have been used to investigate neurons and neuronal networks formation in vitro when guided by artificial nano-scaled cues. Besides, nanotube-based interfaces are being developed, such as prosthesis for monitoring brain activity. We recently described how carbon nanotube substrates alter the electrophysiological and synaptic responses of hippocampal neurons in culture. This observation highlighted the exceptional ability of this material in interfering with nerve tissue growth. Here we test the hypothesis that carbon nanotube scaffolds promote the development of immature neurons isolated from the neonatal rat spinal cord, and maintained in vitro. To address this issue we performed electrophysiological studies associated to gene expression analysis. Our results indicate that spinal neurons plated on electro-conductive carbon nanotubes show a facilitated development. Spinal neurons anticipate the expression of functional markers of maturation, such as the generation of voltage dependent currents or action potentials. These changes are accompanied by a selective modulation of gene expression, involving neuronal and non-neuronal components. Our microarray experiments suggest that carbon nanotube platforms trigger reparative activities involving microglia, in the absence of reactive gliosis. Hence, future tissue scaffolds blended with conductive nanotubes may be exploited to promote cell differentiation and reparative pathways in neural regeneration strategies.
8
8
1
14
e73621
https://doi.org/10.1371/journal.pone.0073621
Fabbro, Alessandra; Sucapane, A.; Toma, Francesca Maria; Calura, E.; Rizzetto, L.; Carrieri, Claudia; Roncaglia, Paola; Martinelli, V.; Scaini, Denis; Masten, L.; Turco, Alessandro; Gustincich, Stefano; Prato, M.; Ballerini, Laura
File in questo prodotto:
File Dimensione Formato  
Fabbro 2013 PLoS One 8 e73621.pdf

accesso aperto

Tipologia: Versione Editoriale (PDF)
Licenza: Creative commons
Dimensione 2.14 MB
Formato Adobe PDF
2.14 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/20.500.11767/14407
Citazioni
  • ???jsp.display-item.citation.pmc??? 17
  • Scopus 49
  • ???jsp.display-item.citation.isi??? 43
social impact