Self-tolerance, a key feature of the immune system, is still a matter of intense debate. We give here evidence for a peculiar behavior of an antiserum against Mycobacterium tuberculosis chaperonin 10 (m-Cpn10), which could have implications for the mechanism of self-recognition by antibodies against non-self. We show that this antiserum can interact in terms of both inhibition of biological activity and physical association (immunoprecipitation), with the mammalian homologue of m-Cpn10, but only if the bacterial protein is present. Several lines of evidence led us to exclude that the two proteins physically associate to form heterocomplexes: (1) the behavior of the antiserum was not shared by a monoclonal antibody against m-Cpn10; (2) a matrix selective for human Cpn10 (h-Cpn10) did not co-purify m-Cpn10; (3) the distribution pattern in non-denaturing isoelectric focusing of labeled m-Cpn10 was not altered by the presence of the unlabeled h-Cpn10. We conclude therefore that the antiserum against M. tuberculosis Cpn10 also recognizes mammalian Cpn10, with an affinity/avidity regulated by the mycobacterial protein, or by the promotion of hetero-oligomerization. This emergence of self-recognition in the presence of M. tuberculosis Cpn10 could imply a breaking of self-tolerance in situations of infection or vaccination.
|Titolo:||Mycobacterial Cpn10 promotes recognition of the mammalian homologue by a mycobacterium-specific antiserum|
|Autori:||Minto, M; Galli, G; Gianazza, E; Eberini, I; Legname, Giuseppe; Fossati, G; Modena, D; Marcucci, F; Mascagni, P; Ghezzi, P; Fratelli, M.|
|Data di pubblicazione:||1998|
|Digital Object Identifier (DOI):||10.1016/S0167-4889(98)00034-2|
|Fulltext via DOI:||https://doi.org/10.1016/S0167-4889(98)00034-2|
|Appare nelle tipologie:||1.1 Journal article|