The chaperonin activity of sequence-related chaperonin 10 proteins requires their aggregation into heptameric structures. We describe size-exclusion chromatography and ultracentrifugation studies that reveal that while Escherichia coli chaperonin 10 exists as a heptamer, the Mycobacterium tuberculosis chaperonin 10 is tetrameric in dilute solutions and in whole M. tuberculosis lysate. At high protein concentration and in the presence of saturating amounts of divalent ions, the protein is heptameric. Human chaperonin 10 is predominantly heptameric, although smaller oligomers were detected. These differences in structural assembly between species may explain differences in biological activity such as antigenicity. Using C-terminal and N-terminal fragments, sequence 1-25 was identified as indispensable for aggregation. CD spectroscopy studies revealed that (i) a minimum at 202-204 nm correlates with aggregation and characterizes not only the spectrum of the mycobacterial protein, but also those of E. coli and human chaperonin 10 proteins; (ii) the interactions between subunits are of the hydrophobic type; and (iii) the anti-parallel beta-pleated sheet is the main secondary structure element of subunits in both tetrameric and heptameric proteins.
Mycobacterium tuberculosis chaperonin 10 forms stable tetrameric and heptameric structures. Implications for its diverse biological activities / Fossati, G; Lucietto, P; Giuliani, P; Coates, Ar; Harding, S; Cölfen, H; Legname, Giuseppe; Chan, E; Zaliani, A; Mascagni, P.. - In: JOURNAL OF BIOLOGICAL CHEMISTRY. - ISSN 1083-351X. - 270:44(1995), pp. 26156-26167. [10.1074/jbc.270.44.26159]
|Titolo:||Mycobacterium tuberculosis chaperonin 10 forms stable tetrameric and heptameric structures. Implications for its diverse biological activities|
|Autori:||Fossati, G; Lucietto, P; Giuliani, P; Coates, Ar; Harding, S; Cölfen, H; Legname, Giuseppe; Chan, E; Zaliani, A; Mascagni, P.|
|Data di pubblicazione:||1995|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1074/jbc.270.44.26159|
|Appare nelle tipologie:||1.1 Journal article|