The present report is the first description of repetitive calcium events generated by discrete ventral spinal neurons maintained in organotypic culture during in vitro maturation stages crucial for network evolution. Ventral interneurons in one-third of slices displayed spontaneous intracellular calcium transients suppressed by calcium-free extracellular solution or by application of cobalt, and resistant to blockers of network activity like TTX, CNQX, APV, strychnine or bicuculline. Our data suggest a primary role for mitochondria in intracellular calcium oscillations, because CCCP, that selectively collapses the mitochondrial electrochemical gradient, eliminated the ability of these neurons to show activity-independent calcium oscillations. Likewise, CGP-37157, a blocker of mitochondrial Na+/Ca2+ exchanger, inhibited oscillations in the majority of neurons. We propose that spontaneous Ca2+ transients, dynamically regulated by mitochondria, occurred in a discrete cluster of interneurons possibly to guide the development of synaptic connections.
|Titolo:||Activity-independent intracellular calcium signals are spontaneously generated in ventral spinal neurons during development in vitro|
|Autori:||FABBRO A.; PASTORE B.; NISTRI A.; BALLERINI L.|
|Data di pubblicazione:||2007|
|Digital Object Identifier (DOI):||10.1016/j.ceca.2006.07.006|
|Appare nelle tipologie:||1.1 Journal article|