Rabbit antibodies raised against dianthin 30, a ribosome inactivating protein from carnation (Dianthus caryophyllus) leaves, were used to identify a full length dianthin precursor cDNA clone from a lambda gt11 expression library. N-terminal amino acid sequencing of purified dianthin 30 and dianthin 32 confirmed that the clone encoded dianthin 30. The cDNA was 1153 basepairs in length and encoded a precursor protein of 293 amino acid residues. The first 23 N-terminal amino acids of the precursor represented the signal sequence. The protein contained a carboxy-terminal region which, by analogy with barley lectin, may contain a vacuolar targeting signal.
|Titolo:||Nucleotide sequence of cDNA coding for dianthin 30, a ribosome inactivating protein from Dianthus caryophyllus|
|Autori:||Legname G; Bellosta P; Gromo G; Modena D; Keen JN; Roberts LM; Lord JM|
|Rivista:||BIOCHIMICA ET BIOPHYSICA ACTA|
|Data di pubblicazione:||1991|
|Appare nelle tipologie:||1.1 Journal article|