Urinary catheters expose patients to a high risk of acquiring nosocomial infections. To prevent this risk of infec- tion, cellobiose dehydrogenase (CDH), an antimicrobial enzyme able to use various oligosaccharides as electron donors to produce hydrogen peroxide using oxygen as an electron acceptor, was covalently grafted onto plasma- activated urinary polydimethylsiloxane (PDMS) catheter surfaces. Successful immobilization of CDH on PDMS was confirmed by Fourier transformed-infrared spectrometry and production of H2O2. The CDH functionalized PDMS surfaces reduced the amount of viable Staphylococcus aureus by 60%, total biomass deposited on the surface by 30% and 70% of biofilm formation. The immobilized CDH was relatively stable in artificial urine over 16 days, retaining 20% of its initial activity. The CDH coated PDMS surface did not affect the growth and physiology of HEK 239 and RAW 264,7 mamma- lian cells. Therefore this new CDH functionalized catheter sys- tem shows great potential for solving the current problems associated with urinary catheters.

Cellobiose dehydrogenase functionalized urinary catheter as novel antibiofilm system / Thallinger, B.; Brandauer, M.; Burger, P.; Sygmund, C.; Ludwig, R.; Ivanova, K.; Kun, J.; Scaini, Denis; Burnet, M.; Tzanov, T.; Nyanhongo, G. S.; Guebitz, G. M.. - In: JOURNAL OF BIOMEDICAL MATERIALS RESEARCH. PART B, APPLIED BIOMATERIALS.. - ISSN 1552-4973. - 104:7(2016), pp. 1448-1456. [10.1002/jbm.b.33491]

Cellobiose dehydrogenase functionalized urinary catheter as novel antibiofilm system

Scaini, Denis;
2016-01-01

Abstract

Urinary catheters expose patients to a high risk of acquiring nosocomial infections. To prevent this risk of infec- tion, cellobiose dehydrogenase (CDH), an antimicrobial enzyme able to use various oligosaccharides as electron donors to produce hydrogen peroxide using oxygen as an electron acceptor, was covalently grafted onto plasma- activated urinary polydimethylsiloxane (PDMS) catheter surfaces. Successful immobilization of CDH on PDMS was confirmed by Fourier transformed-infrared spectrometry and production of H2O2. The CDH functionalized PDMS surfaces reduced the amount of viable Staphylococcus aureus by 60%, total biomass deposited on the surface by 30% and 70% of biofilm formation. The immobilized CDH was relatively stable in artificial urine over 16 days, retaining 20% of its initial activity. The CDH coated PDMS surface did not affect the growth and physiology of HEK 239 and RAW 264,7 mamma- lian cells. Therefore this new CDH functionalized catheter sys- tem shows great potential for solving the current problems associated with urinary catheters.
2016
104
7
1448
1456
http://onlinelibrary.wiley.com/doi/10.1002/jbm.b.33491/pdf
https://www.ncbi.nlm.nih.gov/pubmed/?term=Cellobiose+dehydrogenase+functionalized+urinary+catheter+as+novel+antibiofilm+system
Thallinger, B.; Brandauer, M.; Burger, P.; Sygmund, C.; Ludwig, R.; Ivanova, K.; Kun, J.; Scaini, Denis; Burnet, M.; Tzanov, T.; Nyanhongo, G. S.; Guebitz, G. M.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.11767/33185
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