Since different culturing parameters - such as media composition or cell density - lead to different experimental results, it is important to define the protocol used for neuronal cultures. The vital role of astrocytes in maintaining homeostasis of neurons - both in vivo and in vitro - is well established: the majority of improved culturing conditions for primary dissociated neuronal cultures rely on astrocytes.
An improved method for growing neurons: Comparison with standard protocols / Pozzi, Diletta; Ban, Jelena; Iseppon, Federico; Torre, Vincent. - In: JOURNAL OF NEUROSCIENCE METHODS. - ISSN 0165-0270. - 280:15 March(2017), pp. 1-10. [10.1016/j.jneumeth.2017.01.013]
An improved method for growing neurons: Comparison with standard protocols
Pozzi, Diletta;Ban, Jelena;Iseppon, Federico;Torre, Vincent
2017-01-01
Abstract
Since different culturing parameters - such as media composition or cell density - lead to different experimental results, it is important to define the protocol used for neuronal cultures. The vital role of astrocytes in maintaining homeostasis of neurons - both in vivo and in vitro - is well established: the majority of improved culturing conditions for primary dissociated neuronal cultures rely on astrocytes.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
