During the development of the central nervous system (CNS), combinations of transcription factors and signalling molecules orchestrate patterning, specification and differentiation of neural cell types. In vertebrates, three types of melanin-containing pigment cells, exert a variety of functional roles including visual perception. Here we analysed the mechanisms underlying pigment cell specification within the CNS of a simple chordate, the ascidian Ciona intestinalis. Ciona tadpole larvae exhibit a basic chordate body plan characterized by a small number of neural cells. We employed lineage-specific transcription profiling to characterize the expression of genes downstream of fibroblast growth factor signalling, which govern pigment cell formation. We demonstrate that FGF signalling sequentially imposes a pigment cell identity at the expense of anterior neural fates. We identify FGF-dependent and pigment cell-specific factors, including the small GTPase, Rab32/38 and demonstrated its requirement for the pigmentation of larval sensory organs.

Fibroblast growth factor signalling controls nervous system patterning and pigment cell formation in Ciona intestinalis / Racioppi, Claudia; Kamal, Ashwani K.; Razy-Krajka, Florian; Gambardella, Gennaro; Zanetti, Laura; Di Bernardo, Diego; Sanges, Remo; Christiaen, Lionel A.; Ristoratore, Filomena. - In: NATURE COMMUNICATIONS. - ISSN 2041-1723. - 5(2014), pp. 1-17. [10.1038/ncomms5830]

Fibroblast growth factor signalling controls nervous system patterning and pigment cell formation in Ciona intestinalis

Sanges, Remo
Membro del Collaboration group
;
2014

Abstract

During the development of the central nervous system (CNS), combinations of transcription factors and signalling molecules orchestrate patterning, specification and differentiation of neural cell types. In vertebrates, three types of melanin-containing pigment cells, exert a variety of functional roles including visual perception. Here we analysed the mechanisms underlying pigment cell specification within the CNS of a simple chordate, the ascidian Ciona intestinalis. Ciona tadpole larvae exhibit a basic chordate body plan characterized by a small number of neural cells. We employed lineage-specific transcription profiling to characterize the expression of genes downstream of fibroblast growth factor signalling, which govern pigment cell formation. We demonstrate that FGF signalling sequentially imposes a pigment cell identity at the expense of anterior neural fates. We identify FGF-dependent and pigment cell-specific factors, including the small GTPase, Rab32/38 and demonstrated its requirement for the pigmentation of larval sensory organs.
5
1
17
5830
10.1038/ncomms5830
http://www.nature.com/ncomms/index.html
Racioppi, Claudia; Kamal, Ashwani K.; Razy-Krajka, Florian; Gambardella, Gennaro; Zanetti, Laura; Di Bernardo, Diego; Sanges, Remo; Christiaen, Lionel A.; Ristoratore, Filomena
File in questo prodotto:
File Dimensione Formato  
Racioppi et al. - 2014 - Fibroblast growth factor signalling controls nervo.pdf

accesso aperto

Tipologia: Versione Editoriale (PDF)
Licenza: Creative commons
Dimensione 3.9 MB
Formato Adobe PDF
3.9 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/20.500.11767/68560
Citazioni
  • ???jsp.display-item.citation.pmc??? 20
  • Scopus 34
  • ???jsp.display-item.citation.isi??? 36
social impact