Here, we report the first use of massive-scale RNA-sequencing to explore seagrass response to CO2-driven ocean acidification (OA). Large-scale gene expression changes in the seagrass Cymodocea nodosa occurred at CO2levels projected by the end of the century. C. nodosa transcriptome was obtained using Illumina RNA-Seq technology and de novo assembly, and differential gene expression was explored in plants exposed to short-term high CO2/low pH conditions. At high pCO2, there was a significant increased expression of transcripts associated with photosynthesis, including light reaction functions and CO2fixation, and also to respiratory pathways, specifically for enzymes involved in glycolysis, in the tricarboxylic acid cycle and in the energy metabolism of the mitochondrial electron transport. The upregulation of respiratory metabolism is probably supported by the increased availability of photosynthates and increased energy demand for biosynthesis and stress-related processes under elevated CO2and low pH. The upregulation of several chaperones resembling heat stress-induced changes in gene expression highlighted the positive role these proteins play in tolerance to intracellular acid stress in seagrasses. OA further modifies C. nodosa secondary metabolism inducing the transcription of enzymes related to biosynthesis of carbon-based secondary compounds, in particular the synthesis of polyphenols and isoprenoid compounds that have a variety of biological functions including plant defence. By demonstrating which physiological processes are most sensitive to OA, this research provides a major advance in the understanding of seagrass metabolism in the context of altered seawater chemistry from global climate change.

Genomewide transcriptional reprogramming in the seagrass Cymodocea nodosa under experimental ocean acidification / Ruocco, Miriam; Musacchia, Francesco; Olivé, Irene; Costa, Monya M.; Barrote, Isabel; Santos, Rui; Sanges, Remo; Procaccini, Gabriele; Silva, João. - In: MOLECULAR ECOLOGY. - ISSN 0962-1083. - 26:16(2017), pp. 4241-4259. [10.1111/mec.14204]

Genomewide transcriptional reprogramming in the seagrass Cymodocea nodosa under experimental ocean acidification

Sanges, Remo
Membro del Collaboration group
;
2017

Abstract

Here, we report the first use of massive-scale RNA-sequencing to explore seagrass response to CO2-driven ocean acidification (OA). Large-scale gene expression changes in the seagrass Cymodocea nodosa occurred at CO2levels projected by the end of the century. C. nodosa transcriptome was obtained using Illumina RNA-Seq technology and de novo assembly, and differential gene expression was explored in plants exposed to short-term high CO2/low pH conditions. At high pCO2, there was a significant increased expression of transcripts associated with photosynthesis, including light reaction functions and CO2fixation, and also to respiratory pathways, specifically for enzymes involved in glycolysis, in the tricarboxylic acid cycle and in the energy metabolism of the mitochondrial electron transport. The upregulation of respiratory metabolism is probably supported by the increased availability of photosynthates and increased energy demand for biosynthesis and stress-related processes under elevated CO2and low pH. The upregulation of several chaperones resembling heat stress-induced changes in gene expression highlighted the positive role these proteins play in tolerance to intracellular acid stress in seagrasses. OA further modifies C. nodosa secondary metabolism inducing the transcription of enzymes related to biosynthesis of carbon-based secondary compounds, in particular the synthesis of polyphenols and isoprenoid compounds that have a variety of biological functions including plant defence. By demonstrating which physiological processes are most sensitive to OA, this research provides a major advance in the understanding of seagrass metabolism in the context of altered seawater chemistry from global climate change.
26
16
4241
4259
http://www.blackwellpublishing.com/journals/MEC
Ruocco, Miriam; Musacchia, Francesco; Olivé, Irene; Costa, Monya M.; Barrote, Isabel; Santos, Rui; Sanges, Remo; Procaccini, Gabriele; Silva, João
File in questo prodotto:
File Dimensione Formato  
Ruocco et al. - 2017 - Genomewide transcriptional reprogramming in the se.pdf

non disponibili

Tipologia: Versione Editoriale (PDF)
Licenza: Non specificato
Dimensione 1.24 MB
Formato Adobe PDF
1.24 MB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/20.500.11767/68598
Citazioni
  • ???jsp.display-item.citation.pmc??? 8
  • Scopus 17
  • ???jsp.display-item.citation.isi??? 17
social impact