Microalgae play a major role as primary producers in aquatic ecosystems. Cell signalling regulates their interactions with the environment and other organisms, yet this process in phytoplankton is poorly defined. Using the marine planktonic diatom Pseudo-nitzschia multistriata, we investigated the cell response to cues released during sexual reproduction, an event that demands strong regulatory mechanisms and impacts on population dynamics. We sequenced the genome of P. multistriata and performed phylogenomic and transcriptomic analyses, which allowed the definition of gene gains and losses, horizontal gene transfers, conservation and evolutionary rate of sex-related genes. We also identified a small number of conserved noncoding elements. Sexual reproduction impacted on cell cycle progression and induced an asymmetric response of the opposite mating types. G protein-coupled receptors and cyclic guanosine monophosphate (cGMP) are implicated in the response to sexual cues, which overall entails a modulation of cell cycle, meiosis-related and nutrient transporter genes, suggesting a fine control of nutrient uptake even under nutrient-replete conditions. The controllable life cycle and the genome sequence of P. multistriata allow the reconstruction of changes occurring in diatoms in a key phase of their life cycle, providing hints on the evolution and putative function of their genes and empowering studies on sexual reproduction.

Finding a partner in the ocean: molecular and evolutionary bases of the response to sexual cues in a planktonic diatom / Basu, Swaraj; Patil, Shrikant; Mapleson, Daniel; Russo, Monia Teresa; Vitale, Laura; Fevola, Cristina; Maumus, Florian; Casotti, Raffaella; Mock, Thomas; Caccamo, Mario; Montresor, Marina; Sanges, Remo; Ferrante, Maria Immacolata. - In: NEW PHYTOLOGIST. - ISSN 0028-646X. - 215:1(2017), pp. 140-156. [10.1111/nph.14557]

Finding a partner in the ocean: molecular and evolutionary bases of the response to sexual cues in a planktonic diatom

Sanges, Remo
Supervision
;
2017-01-01

Abstract

Microalgae play a major role as primary producers in aquatic ecosystems. Cell signalling regulates their interactions with the environment and other organisms, yet this process in phytoplankton is poorly defined. Using the marine planktonic diatom Pseudo-nitzschia multistriata, we investigated the cell response to cues released during sexual reproduction, an event that demands strong regulatory mechanisms and impacts on population dynamics. We sequenced the genome of P. multistriata and performed phylogenomic and transcriptomic analyses, which allowed the definition of gene gains and losses, horizontal gene transfers, conservation and evolutionary rate of sex-related genes. We also identified a small number of conserved noncoding elements. Sexual reproduction impacted on cell cycle progression and induced an asymmetric response of the opposite mating types. G protein-coupled receptors and cyclic guanosine monophosphate (cGMP) are implicated in the response to sexual cues, which overall entails a modulation of cell cycle, meiosis-related and nutrient transporter genes, suggesting a fine control of nutrient uptake even under nutrient-replete conditions. The controllable life cycle and the genome sequence of P. multistriata allow the reconstruction of changes occurring in diatoms in a key phase of their life cycle, providing hints on the evolution and putative function of their genes and empowering studies on sexual reproduction.
2017
215
1
140
156
10.1111/nph.14557
http://www.wiley.com/bw/editors.asp?ref=0028-646X&site=1
Basu, Swaraj; Patil, Shrikant; Mapleson, Daniel; Russo, Monia Teresa; Vitale, Laura; Fevola, Cristina; Maumus, Florian; Casotti, Raffaella; Mock, Thomas; Caccamo, Mario; Montresor, Marina; Sanges, Remo; Ferrante, Maria Immacolata
File in questo prodotto:
File Dimensione Formato  
Basu et al. - 2017 - Finding a partner in the ocean molecular and evol.pdf

accesso aperto

Tipologia: Versione Editoriale (PDF)
Licenza: Creative commons
Dimensione 2.45 MB
Formato Adobe PDF
2.45 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.11767/68616
Citazioni
  • ???jsp.display-item.citation.pmc??? 37
  • Scopus 86
  • ???jsp.display-item.citation.isi??? 74
social impact