Following implantation, neuroelectrode functionality is susceptible to deterioration via reactive host cell response and glial scar-induced encapsulation. Within the neuroengineering community, there is a consensus that the induction of selective adhesion and regulated cellular interaction at the tissue–electrode interface can significantly enhance device interfacing and functionality in vivo. In particular, topographical modification holds promise for the development of functionalized neural interfaces to mediate initial cell adhesion and the subsequent evolution of gliosis, minimizing the onset of a proinflammatory glial phenotype, to provide long-term stability. Herein, a low-temperature microimprint-lithography technique for the development of micro-topographically functionalized neuroelectrode interfaces in electrodeposited poly(3,4-ethylenedioxythiophene):p-toluene sulfonate (PEDOT:PTS) is described and assessed in vitro. Platinum (Pt) microelectrodes are subjected to electrodeposition of a PEDOT:PTS microcoating, which is subsequently topographically functionalized with an ordered array of micropits, inducing a significant reduction in electrode electrical impedance and an increase in charge storage capacity. Furthermore, topographically functionalized electrodes reduce the adhesion of reactive astrocytes in vitro, evident from morphological changes in cell area, focal adhesion formation, and the synthesis of proinflammatory cytokines and chemokine factors. This study contributes to the understanding of gliosis in complex primary mixed cell cultures, and describes the role of micro-topographically modified neural interfaces in the development of stable microelectrode interfaces.

Attenuated Glial Reactivity on Topographically Functionalized Poly(3,4-Ethylenedioxythiophene):P-Toluene Sulfonate (PEDOT:PTS) Neuroelectrodes Fabricated by Microimprint Lithography / Vallejo-Giraldo, Catalina; Krukiewicz, Katarzyna; Calaresu, Ivo; Zhu, Jingyuan; Palma, Matteo; Fernandez-Yague, Marc; Mcdowell, Benjaminw.; Peixoto, Nathalia; Farid, Nazar; O'Connor, Gerard; Ballerini, Laura; Pandit, Abhay; Biggs, Manus Jonathan Paul. - In: SMALL. - ISSN 1613-6810. - 14:28(2018), pp. 1-20. [10.1002/smll.201800863]

Attenuated Glial Reactivity on Topographically Functionalized Poly(3,4-Ethylenedioxythiophene):P-Toluene Sulfonate (PEDOT:PTS) Neuroelectrodes Fabricated by Microimprint Lithography

Calaresu, Ivo
Investigation
;
Ballerini, Laura;
2018

Abstract

Following implantation, neuroelectrode functionality is susceptible to deterioration via reactive host cell response and glial scar-induced encapsulation. Within the neuroengineering community, there is a consensus that the induction of selective adhesion and regulated cellular interaction at the tissue–electrode interface can significantly enhance device interfacing and functionality in vivo. In particular, topographical modification holds promise for the development of functionalized neural interfaces to mediate initial cell adhesion and the subsequent evolution of gliosis, minimizing the onset of a proinflammatory glial phenotype, to provide long-term stability. Herein, a low-temperature microimprint-lithography technique for the development of micro-topographically functionalized neuroelectrode interfaces in electrodeposited poly(3,4-ethylenedioxythiophene):p-toluene sulfonate (PEDOT:PTS) is described and assessed in vitro. Platinum (Pt) microelectrodes are subjected to electrodeposition of a PEDOT:PTS microcoating, which is subsequently topographically functionalized with an ordered array of micropits, inducing a significant reduction in electrode electrical impedance and an increase in charge storage capacity. Furthermore, topographically functionalized electrodes reduce the adhesion of reactive astrocytes in vitro, evident from morphological changes in cell area, focal adhesion formation, and the synthesis of proinflammatory cytokines and chemokine factors. This study contributes to the understanding of gliosis in complex primary mixed cell cultures, and describes the role of micro-topographically modified neural interfaces in the development of stable microelectrode interfaces.
14
28
1
20
1800863
10.1002/smll.201800863
http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1613-6829
Vallejo-Giraldo, Catalina; Krukiewicz, Katarzyna; Calaresu, Ivo; Zhu, Jingyuan; Palma, Matteo; Fernandez-Yague, Marc; Mcdowell, Benjaminw.; Peixoto, Nathalia; Farid, Nazar; O'Connor, Gerard; Ballerini, Laura; Pandit, Abhay; Biggs, Manus Jonathan Paul
File in questo prodotto:
File Dimensione Formato  
2018 Small.pdf

non disponibili

Descrizione: main text
Tipologia: Versione Editoriale (PDF)
Licenza: Non specificato
Dimensione 3.9 MB
Formato Adobe PDF
3.9 MB Adobe PDF   Visualizza/Apri   Richiedi una copia
Vallejo-Giraldo et al-smll.201800863_R1-1.pdf

embargo fino al 04/06/2019

Tipologia: Documento in Post-print
Licenza: Non specificato
Dimensione 4.31 MB
Formato Adobe PDF
4.31 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.11767/81467
Citazioni
  • ???jsp.display-item.citation.pmc??? 4
  • Scopus 18
  • ???jsp.display-item.citation.isi??? 19
social impact