A broad diversity of sex-determining systems has evolved in eukaryotes. However, information on the mechanisms of sex determination for unicellular microalgae is limited, including for diatoms, key-players of ocean food webs. Here we report the identification of a mating type (MT) determining gene for the diatom Pseudo-nitzschia multistriata. By comparing the expression profile of the two MTs, we find five MT-biased genes, of which one, MRP3, is expressed exclusively in MT+ strains in a monoallelic manner. A short tandem repeat of specific length in the region upstream of MRP3 is consistently present in MT+ and absent in MT- strains. MRP3 overexpression in an MT- strain induces sex reversal: the transgenic MT- can mate with another MT- strain and displays altered regulation of the other MT-biased genes, indicating that they lie downstream. Our data show that a relatively simple genetic program is involved in defining the MT in P. multistriata.

MRP3 is a sex determining gene in the diatom Pseudo-nitzschia multistriata / Russo, Monia T; Vitale, Laura; Entrambasaguas, Laura; Anestis, Konstantinos; Fattorini, Neri; Romano, Filomena; Minucci, Carmen; De Luca, Pasquale; Biffali, Elio; Vyverman, Wim; Sanges, Remo; Montresor, Marina; Ferrante, Maria I. - In: NATURE COMMUNICATIONS. - ISSN 2041-1723. - 9:1(2018), pp. 1-10. [10.1038/s41467-018-07496-0]

MRP3 is a sex determining gene in the diatom Pseudo-nitzschia multistriata

Sanges, Remo
Membro del Collaboration group
;
2018

Abstract

A broad diversity of sex-determining systems has evolved in eukaryotes. However, information on the mechanisms of sex determination for unicellular microalgae is limited, including for diatoms, key-players of ocean food webs. Here we report the identification of a mating type (MT) determining gene for the diatom Pseudo-nitzschia multistriata. By comparing the expression profile of the two MTs, we find five MT-biased genes, of which one, MRP3, is expressed exclusively in MT+ strains in a monoallelic manner. A short tandem repeat of specific length in the region upstream of MRP3 is consistently present in MT+ and absent in MT- strains. MRP3 overexpression in an MT- strain induces sex reversal: the transgenic MT- can mate with another MT- strain and displays altered regulation of the other MT-biased genes, indicating that they lie downstream. Our data show that a relatively simple genetic program is involved in defining the MT in P. multistriata.
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Russo, Monia T; Vitale, Laura; Entrambasaguas, Laura; Anestis, Konstantinos; Fattorini, Neri; Romano, Filomena; Minucci, Carmen; De Luca, Pasquale; Biffali, Elio; Vyverman, Wim; Sanges, Remo; Montresor, Marina; Ferrante, Maria I
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/20.500.11767/85978
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