Fluorescent protein reporters have become the mainstay for tracing cellular circuitry in vivo but are limited in their versatility. Here we generated Cre-dependent reporter mice expressing the Snap-tag to target synthetic indicators to cells. Snap-tag labeling worked efficiently and selectively in vivo, allowing for both the manipulation of behavior and monitoring of cellular fluorescence from the same reporter.

Genetic targeting of chemical indicators in vivo / Yang, Guoying; De Castro Reis, Fernanda; Sundukova, Mayya; Pimpinella, Sofia; Asaro, Antonino; Castaldi, Laura; Batti, Laura; Bilbao, Daniel; Reymond, Luc; Johnsson, Kai; Heppenstall, Paul A.. - In: NATURE METHODS. - ISSN 1548-7091. - 12:2(2015), pp. 137-139. [10.1038/nmeth.3207]

Genetic targeting of chemical indicators in vivo

Sundukova, Mayya;Heppenstall, Paul A.
2015

Abstract

Fluorescent protein reporters have become the mainstay for tracing cellular circuitry in vivo but are limited in their versatility. Here we generated Cre-dependent reporter mice expressing the Snap-tag to target synthetic indicators to cells. Snap-tag labeling worked efficiently and selectively in vivo, allowing for both the manipulation of behavior and monitoring of cellular fluorescence from the same reporter.
12
2
137
139
https://www.nature.com/articles/nmeth.3207
Yang, Guoying; De Castro Reis, Fernanda; Sundukova, Mayya; Pimpinella, Sofia; Asaro, Antonino; Castaldi, Laura; Batti, Laura; Bilbao, Daniel; Reymond, Luc; Johnsson, Kai; Heppenstall, Paul A.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/20.500.11767/87778
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