White matter (WM) plasticity during adulthood is a recently described phenomenon by which experience can shape brain structure. It has been observed in humans using diffusion tensor imaging (DTI) and myelination has been suggested as a possible mechanism. Here, we set out to identify molecular and cellular changes associated with WM plasticity measured by DTI. We combined DTI, immunohistochemistry and mRNA expression analysis and examined the effects of somatosensory experience in adult rats. First, we observed experience-induced DTI differences in WM and in grey matter structure. C-Fos mRNA expression, a marker of cortical activity, in the barrel cortex correlated with the MRI WM metrics, indicating that molecular correlates of cortical activity relate to macroscale measures of WM structure. Analysis of myelin-related genes revealed higher myelin basic protein (MBP) mRNA expression. Higher MBP protein expression was also found via immunohistochemistry in WM. Finally, unbiased RNA sequencing analysis identified 134 differentially expressed genes encoding proteins in- volved in functions related to cell proliferation and differentiation, regulation of myelination and neuronal activity modulation. In conclusion, macroscale measures of WM plasticity are supported by both molecular and cellular evidence and confirm that myelination is one of the underlying mechanisms.

White matter structure and myelin-related gene expression alterations with experience in adult rats / Sampaio-Baptista, C.; Vallès, A.; Khrapitchev, A. A.; Akkermans, G.; Winkler, A. M.; Foxley, S.; Sibson, N. R.; Roberts, M.; Miller, K.; Diamond, M. E.; Martens, G. J. M.; De Weerd, P.; Johansen-Berg, H. - In: PROGRESS IN NEUROBIOLOGY. - ISSN 0301-0082. - 187:(2020), pp. 1-13. [10.1016/j.pneurobio.2020.101770]

White matter structure and myelin-related gene expression alterations with experience in adult rats

Diamond, M. E.
Membro del Collaboration group
;
2020-01-01

Abstract

White matter (WM) plasticity during adulthood is a recently described phenomenon by which experience can shape brain structure. It has been observed in humans using diffusion tensor imaging (DTI) and myelination has been suggested as a possible mechanism. Here, we set out to identify molecular and cellular changes associated with WM plasticity measured by DTI. We combined DTI, immunohistochemistry and mRNA expression analysis and examined the effects of somatosensory experience in adult rats. First, we observed experience-induced DTI differences in WM and in grey matter structure. C-Fos mRNA expression, a marker of cortical activity, in the barrel cortex correlated with the MRI WM metrics, indicating that molecular correlates of cortical activity relate to macroscale measures of WM structure. Analysis of myelin-related genes revealed higher myelin basic protein (MBP) mRNA expression. Higher MBP protein expression was also found via immunohistochemistry in WM. Finally, unbiased RNA sequencing analysis identified 134 differentially expressed genes encoding proteins in- volved in functions related to cell proliferation and differentiation, regulation of myelination and neuronal activity modulation. In conclusion, macroscale measures of WM plasticity are supported by both molecular and cellular evidence and confirm that myelination is one of the underlying mechanisms.
187
1
13
101770
https://doi.org/10.1016/j.pneurobio.2020.101770
Sampaio-Baptista, C.; Vallès, A.; Khrapitchev, A. A.; Akkermans, G.; Winkler, A. M.; Foxley, S.; Sibson, N. R.; Roberts, M.; Miller, K.; Diamond, M. E.; Martens, G. J. M.; De Weerd, P.; Johansen-Berg, H
File in questo prodotto:
File Dimensione Formato  
Sampaio-Baptista et al. (2020)_Progr. in Neurobiol.pdf

accesso aperto

Tipologia: Versione Editoriale (PDF)
Licenza: Creative commons
Dimensione 5.11 MB
Formato Adobe PDF
5.11 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.11767/108916
Citazioni
  • ???jsp.display-item.citation.pmc??? 11
  • Scopus 21
  • ???jsp.display-item.citation.isi??? 20
social impact