Mammalian genomes encode numerous natural antisense long noncoding RNAs (lncRNAs) that regulate gene expression. Recently, an antisense lncRNA to mouse Ubiquitin carboxyl-terminal hydrolase L1 (Uchl1) was reported to increase UCHL1 protein synthesis, representing a new functional class of lncRNAs, designated as SINEUPs, for SINE element-containing translation UP-regulators. Here, we show that an antisense lncRNA to the human protein phosphatase 1 regulatory subunit 12A (PPP1R12A), named as R12A-AS1, which overlaps with the 5' UTR and first coding exon of the PPP1R12A mRNA, functions as a SINEUP, increasing PPP1R12A protein translation in human cells. The SINEUP activity depends on the aforementioned sense-antisense interaction and a free right Alu monomer repeat element at the 3' end of R12A-AS1. In addition, we identify another human antisense lncRNA with SINEUP activity. Our results demonstrate for the first time that human natural antisense lncRNAs can up-regulate protein translation, suggesting that endogenous SINEUPs may be widespread and present in many mammalian species.
|Titolo:||Identification of antisense long noncoding RNAs that function as SINEUPs in human cells|
|Autori:||Schein A.; Zucchelli S.; Kauppinen S.; Gustincich S.; Carninci P.|
|Data di pubblicazione:||2016|
|Numero di Articolo:||33605|
|Digital Object Identifier (DOI):||10.1038/srep33605|
|Fulltext via DOI:||10.1038/srep33605|
|Appare nelle tipologie:||1.1 Journal article|