In recent years the research on the olfactory system has entered a phase of deep innovation, regardless of the animal model taken as a reference. While the advancements achieved in different fields have provided answer to old questions, the striking evidences that have emerged in this new olfactory landscape have brought new ideas, new hypothesis and new scientific problems that necessarily need to be approached with adequate tools and strategies. The work presented in this thesis has targeted three different issues among the more intriguing ones concerning the murine olfactory system. The project described in the first section has conf ronted with the molecular identity of the Calcium-activated chloride channel responsible for the amplification of cationic currents in olfactory sensory neurons, a key mechanism for the triggering of action potentials after binding of odour molecules with their specific receptors. Olfactory microvillar cells constitute a cell population largely represented in the main olfactory epithelium, but their role is still poorly understood mostly because a precise genomic characterization of this cell-type has never been undertaken; the project presented in the second section has tried to reveal the genomic fingerprint of microvillar cells through a custom gene expression profiling. The data presented in the third section of this thesis are the result of a deep genomic investigation that has targeted the entire transcriptome of the olfactory sensory epithelium exploiting a newly developed high-throughput tagging approach derived from the Cap-Analysis of Gene Expression (CAGE) technology. The potential of this workflow has allowed revealing new details about the expression of pheromone vomeronasal receptors in the main olfactory epithelium.

Targeting the complexity of mouse olfactory system / Pascarella, Giovanni. - (2008 Oct 31).

Targeting the complexity of mouse olfactory system

Pascarella, Giovanni
2008-10-31

Abstract

In recent years the research on the olfactory system has entered a phase of deep innovation, regardless of the animal model taken as a reference. While the advancements achieved in different fields have provided answer to old questions, the striking evidences that have emerged in this new olfactory landscape have brought new ideas, new hypothesis and new scientific problems that necessarily need to be approached with adequate tools and strategies. The work presented in this thesis has targeted three different issues among the more intriguing ones concerning the murine olfactory system. The project described in the first section has conf ronted with the molecular identity of the Calcium-activated chloride channel responsible for the amplification of cationic currents in olfactory sensory neurons, a key mechanism for the triggering of action potentials after binding of odour molecules with their specific receptors. Olfactory microvillar cells constitute a cell population largely represented in the main olfactory epithelium, but their role is still poorly understood mostly because a precise genomic characterization of this cell-type has never been undertaken; the project presented in the second section has tried to reveal the genomic fingerprint of microvillar cells through a custom gene expression profiling. The data presented in the third section of this thesis are the result of a deep genomic investigation that has targeted the entire transcriptome of the olfactory sensory epithelium exploiting a newly developed high-throughput tagging approach derived from the Cap-Analysis of Gene Expression (CAGE) technology. The potential of this workflow has allowed revealing new details about the expression of pheromone vomeronasal receptors in the main olfactory epithelium.
31-ott-2008
Gustincich, Stefano
Pascarella, Giovanni
File in questo prodotto:
File Dimensione Formato  
1963_6170_PhD_Giovanni_Pascarella.pdf

accesso aperto

Tipologia: Tesi
Licenza: Non specificato
Dimensione 6.64 MB
Formato Adobe PDF
6.64 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.11767/4677
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact