The use of graphenebased materials to engineer sophisticated biosensing interfaces that can adapt to the central nervous system requires a detailed understanding of how such materials behave in a biological context. Graphene's peculiar properties can cause various cellular changes, but the underlying mechanisms remain unclear. Here, we show that singlelayer graphene increases neuronal firing by altering membraneassociated functions in cultured cells. Graphene tunes the distribution of extracellular ions at the interface with neurons, a key regulator of neuronal excitability. The resulting biophysical changes in the membrane include stronger potassium ion currents, with a shift in the fraction of neuronal firing phenotypes from adapting to tonically firing. By using experimental and theoretical approaches, we hypothesize that the graphene–ion interactions that are maximized when singlelayer graphene is deposited on electrically insulating substrates are crucial to these effects.

Single-layer graphene modulates neuronal communication and augments membrane ion currents / Pampaloni, Niccolò Paolo; Lottner, Martin; Giugliano, Michele; Matruglio, Alessia; D’Amico, Francesco; Prato, Maurizio; Antonio Garrido, Josè; Ballerini, Laura; Scaini, Denis. - In: NATURE NANOTECHNOLOGY. - ISSN 1748-3395. - 13:8(2018), pp. 755-764. [10.1038/s41565-018-0163-6]

Single-layer graphene modulates neuronal communication and augments membrane ion currents

Niccolò Paolo Pampaloni
Investigation
;
Michele Giugliano
Formal Analysis
;
Laura Ballerini
Conceptualization
;
Denis Scaini
Conceptualization
2018

Abstract

The use of graphenebased materials to engineer sophisticated biosensing interfaces that can adapt to the central nervous system requires a detailed understanding of how such materials behave in a biological context. Graphene's peculiar properties can cause various cellular changes, but the underlying mechanisms remain unclear. Here, we show that singlelayer graphene increases neuronal firing by altering membraneassociated functions in cultured cells. Graphene tunes the distribution of extracellular ions at the interface with neurons, a key regulator of neuronal excitability. The resulting biophysical changes in the membrane include stronger potassium ion currents, with a shift in the fraction of neuronal firing phenotypes from adapting to tonically firing. By using experimental and theoretical approaches, we hypothesize that the graphene–ion interactions that are maximized when singlelayer graphene is deposited on electrically insulating substrates are crucial to these effects.
13
8
755
764
https://doi.org/10.1038/s41565-018-0163-6
Pampaloni, Niccolò Paolo; Lottner, Martin; Giugliano, Michele; Matruglio, Alessia; D’Amico, Francesco; Prato, Maurizio; Antonio Garrido, Josè; Ballerini, Laura; Scaini, Denis
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/20.500.11767/76774
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