Bitter and sweet receptors (T2Rs and T1Rs) are expressed in many extra-oral tissues including upper and lower airways. To investigate if bitter tastants and artificial sweeteners could activate physiological responses in tracheal epithelial cells we performed confocal Ca2+ imaging recordings on acute tracheal slices. We stimulated the cells with denatonium benzoate, a T2R agonist, and with the artificial sweeteners sucralose, saccharin and acesulfame-K. To test cell viability we measured responses to ATP. We found that 39% of the epithelial cells responding to ATP also responded to bitter stimulation with denatonium benzoate. Moreover, artificial sweeteners activated different percentages of the cells, ranging from 5% for sucralose to 26% for saccharin, and 27% for acesulfame-K. By using carbenoxolone, a gap junction blocker, we excluded that responses were mainly mediated by Ca2+ waves through cell-to-cell junctions. Pharmacological experiments showed that both denatonium and artificial sweeteners induced a PLC-mediated release of Ca2+ from internal stores. In addition, bitter tastants and artificial sweeteners activated a partially overlapping subpopulation of tracheal epithelial cells. Our results provide new evidence that a subset of ATP-responsive tracheal epithelial cells from rat are activated by both bitter tastants and artificial sweeteners.
|Titolo:||Bitter tastants and artificial sweeteners activate a subset of epithelial cells in acute tissue slices of the rat trachea|
|Autori:||Lasconi, Chiara; Pifferi, Simone; Hernandez-Clavijo, Andres; Merigo, Flavia; Cecchini Maria, Paola; Gonzalez-Velandia Kevin, Y.; Agostinelli, Emilio; Sbarbati, Andrea; Menini, Anna|
|Data di pubblicazione:||2019|
|Numero di Articolo:||8834|
|Digital Object Identifier (DOI):||10.1038/s41598-019-45456-w|
|Appare nelle tipologie:||1.1 Journal article|